TABLE 2:

Summary of immunohistochemical and molecular findings in cohort of patients with hybrid schwannoma-perineurioma.

Case	Immunohistochemistry							RNA-seq	FISH
	S100	SOX10	CD34	EMA	Claudin-1	GLUT1	H3K27me3
Index	D+	D+	D+	M+	M+	M+	Intact	CHD7-VGLL3	+ / +
2	D+	D+	M+	M+	N/A	N/A	Intact	CHD7-VGLL3	N/A
3	D+	D+	D+	M+	M+	M+	N/A	CHD7-VGLL3	N/A
4	D+	N/A	M+	M+	Multifocal	N/A	N/A	CHD7-VGLL3	N/A
5	D+	N/A	M+	D+	N/A	N/A	N/A	CHD7-VGLL3	N/A
6	D+	N/A	D+	D+	N/A	N/A	N/A	CHD7-VGLL3	N/A
7	M+	N/A	N/A	M+	N/A	N/A	N/A	CHD7-VGLL3	N/A
8	D+	D+	N/A	N/A	N/A	N/A	N/A	CHD7-VGLL3*	+ / +
9	D+	D+	M+	N/A	N/A	N/A	Intact	CHD7-VGLL3*	+ / +
10	D+	D+	D+	M+	N/A	M+	Intact	CHD7-VGLL3*	+ / +
11	D+	D+	D+	F+	D+	F+	N/A	CHD9-VGLL3	N/A
12	M+	N/A	D+	D+	N/A	N/A	N/A	CHD9-VGLL3	N/A
13	M+	M+	D+	M+	N/A	N/A	Intact	MAMLD1-VGLL3	N/A
14	D+	D+	N/A	−	N/A	N/A	N/A	MAMLD1-VGLL3*	N/A / +
15	M+	M+	M+	M+	M+	M+	N/A	DST-BRAF	+ / +
16	D+	D+	−	M+	M+	−	N/A	SQSTM1-CDX1	N/A
17	M+	N/A	M+	M+	M+	N/A	N/A	Negative	− / −
18	D+	N/A	N/A	M+	M+	N/A	N/A	Negative	− / −

Abbreviations: EMA: epithelial membrane antigen; FISH: fluorescence in situ hybridization; N/A: not assessed; RNA-Seq: RNA sequencing; Quantification of immunohistochemistry: D+ diffusely positive, F+ focal positivity, M+ multifocal, − negative.

^*RNA-Seq performed using Archer platform (all other cases Illumina TruSight RNA Fusion platform).