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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Cell Tissue Res. 2021 Jan 26;384(2):367–387. doi: 10.1007/s00441-020-03379-3

Fig. 6. The differential expression of elastin and CD73 in paratenon, IFM, and fascicles.

Fig. 6

Each component is analyzed in cross (a-d) and longitudinal sections (e-h) staining for elastin (green) and CD73 (red) by immunofluorescent staining. Each component is labeled in a with white boundaries designating specific regions of the tendon. Each component is replicated in a-d images and in e-h images. Paratenon tissues are positively stained with both elastin and CD73 (a-d). The interior of blood vessel-like tissues within the IFM are positively stained by elastin (yellow arrow e, h), with the exterior positively stained for CD73 (yellow arrows in f, h). High levels of net-like IFM are positively stained by elastin (a, c, e, g, h) and CD73 (b, c, f-h), whereas fascicles show a minimal amount of staining for elastin and are negative for CD73 (white arrows in b, c, f, g). Image d is the enlarged blue box area of image c. Image h is the enlarged pink box area of image g. Semi-quantification is displayed in figure i. **p < 0.001 (fascicles compared to both paratenon and IFM). Negative staining images were generated for elastin and CD73 antibodies, as described in the methods. Control images did not show any real signal, with only minor background noise (data not shown). White bars: 100 μm; Pink bars: 25 μm.