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. Author manuscript; available in PMC: 2022 May 1.

Published in final edited form as: Cell Tissue Res. 2021 Jan 26;384(2):367–387. doi: 10.1007/s00441-020-03379-3

Fig. 8 — Cross (a-e) and longitudinal (g-i) tissue sections are stained for CD146 (red fluorescence) and collagen I (green fluorescence), compared to Hoechst H33342 staining (h) and phase contrast images (h). Cross sections show paratenon tissues are positively stained for both CD146 (a) and collagen I (a), IFM also is stained for CD146 and collagen I (a-e), and fascicles are positive for collagen I (a, c-e). Semi-quantification of a-e is displayed in f. *p < 0.001 (IFM compared to paratenon); **p < 0.001 (fascicles compared to both paratenon and IFM). Additional longitudinal tissue sections (g-i) show the IFM (the areas between white dash lines) positively stained for CD146, but very few cells in fascicles are positively stained by CD146 (white arrows). Thus, fascicles are only positively stained by collagen I (yellow arrows in b-e). The image e is the enlarged blue box area within image d which shows that IFM tissues are stained positively by CD146 and fascicles are positively stained by collagen I. White bars: 100 μm; Yellow bar: 25 m.

Inline graphic — Cross (a-e) and longitudinal (g-i) tissue sections are stained for CD146 (red fluorescence) and collagen I (green fluorescence), compared to Hoechst H33342 staining (h) and phase contrast images (h). Cross sections show paratenon tissues are positively stained for both CD146 (a) and collagen I (a), IFM also is stained for CD146 and collagen I (a-e), and fascicles are positive for collagen I (a, c-e). Semi-quantification of a-e is displayed in f. *p < 0.001 (IFM compared to paratenon); **p < 0.001 (fascicles compared to both paratenon and IFM). Additional longitudinal tissue sections (g-i) show the IFM (the areas between white dash lines) positively stained for CD146, but very few cells in fascicles are positively stained by CD146 (white arrows). Thus, fascicles are only positively stained by collagen I (yellow arrows in b-e). The image e is the enlarged blue box area within image d which shows that IFM tissues are stained positively by CD146 and fascicles are positively stained by collagen I. White bars: 100 μm; Yellow bar: 25 m.