Male ND Cyp2b-null 0-PFOS (A), Male ND hCYP2B6 0-PFOS (B), Male HFD hCYP2B6 0-PFOS (C), Female ND Cyp2b-null 0-PFOS (D), Female ND hCYP2B6 0-PFOS (E), Female HFD Cyp2b-null 0-PFOS (F), Female HFD hCYP2B6 0-PFOS (G), and quantified using Image J Fiji Particle Analysis (H). Images were taken at 400x (0.05mm) magnification. Data are presented as mean ± SEM (n = 3). Statistical significance was determined by one-way ANOVA followed by Fisher's LSD as the post-hoc test. ‘p’ indicates difference between PFOS concentrations, ‘g’ indicates difference between gender, ‘d’ indicates difference between diets, ‘b’ indicated difference between genotypes. A letter indicates a p < 0.05, letter w/ * indicates p < 0.01, letter w/ ** indicates p < 0.001 and a letter w/ *** indicates p < 0.0001.