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. Author manuscript; available in PMC: 2022 May 24.
Published in final edited form as: Curr Biol. 2021 Mar 24;31(10):2140–2154.e6. doi: 10.1016/j.cub.2021.02.061

Figure 4. LRRK2-G2019S disrupts acidification of axonal AVs.

Figure 4.

(A) Kymographs of mCherry-EGFP-LC3 vesicles in the proximal axon of WT or G2019S KI mouse cortical neurons. Magenta arrowheads: mCherry-positive traces; green arrowheads: EGFP-positive traces; white arrowheads: mCherry- and EGFP-positive traces. (B-D) Percentage of acidified (= mCherry-only positive) AVs in (B) the distal (mean ± SEM; n = 17-20 neurons from 4 independent cultures; ns, not significant, p=0.55; Mann-Whitney test), (C) the proximal axon of WT and G2019S KI neurons (mean ± SEM; n = 29-30 neurons from 4 independent cultures; **p=0.0084; Mann-Whitney test), (D) the proximal axon of G2019S KI neurons treated with DMSO or 100 nM MLi-2 overnight (mean ± SEM; n = 24-26 neurons from 3 independent cultures; *p=0.0123; Mann-Whitney test).

For acidification of axonal LAMP1 vesicles see Figure S5.