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. 2021 May 26;11:11034. doi: 10.1038/s41598-021-90639-z

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© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differences in pNfL concentrations after different freezing intervals. Bland–Altman plot showing the differences in sNfL concentration between two groups of immediate processing and delayed freezing, (a) freezing after 10 min compared to 3 days at room temperature, (b) freezing after 10 min compared to 3 days at 4–8 °C, (c) freezing after 10 min compared to 7 days at room temperature. Single dots represent samples at two conditions with their mean concentrations on the x-axis and their difference in concentration on the y-axis. The three horizontal lines represent the mean difference (middle) and the mean difference plus 1.96xSD of that difference (upper) and mean difference minus 1.96xSD of that difference (lower).