Table 2.
Comparison of NfL concentrations at different freezing intervals.
| [sNfL] freezing interval: immediate | [sNfL] freezing interval: 3 days RT | [sNfL] freezing interval: 3 days 4–8 °C | [sNfL] freezing interval: 7 days RT | p-valuea | |||||
|---|---|---|---|---|---|---|---|---|---|
| Mean (pg/mL) | 95% CI | Mean (pg/mL) | 95% CI | Mean (pg/mL) | 95% CI | Mean (pg/mL) | 95% CI | ||
| (a) | |||||||||
| Whole cohort | 14.6 | 9.7–19.5 | 15.5 | 9.8–21.2 | 16.0 | 10.3–21.8 | 15.5 | 10.1–20.9 | 0.192 |
| Lowest quartile | 5.2 | 4.8–5.6 | 5.3 | 4.4–6.1 | 5.4 | 4.9–5.4 | 5.6 | 4.9–6.4 | 0.633 |
| Highest quartile | 34.2 | 22.6–45.7 | 36.8 | 20.8–52.7 | 38.5 | 23.7–53.3 | 36.8 | 23.5–50.1 | 0.422 |
| [pNfL] freezing interval: immediate | [pNfL] freezing interval: 3 days RT | [pNfL] freezing interval: 3 days 4–8 °C | [pNfL] freezing interval: 7 days RT | p-valuea | |||||
|---|---|---|---|---|---|---|---|---|---|
| Mean (pg/mL) | 95% CI | Mean (pg/mL) | 95% CI | Mean (pg/mL) | 95% CI | Mean (pg/mL) | 95% CI | ||
| (b) | |||||||||
| Whole cohort | 12.0 | 8.1–15.9 | 12.5 | 8.3–16.8 | 12.8 | 8.1–17.5 | 12.5 | 8.4–16.6 | 0.385 |
| Lowest quartile | 4.8 | 4.1–5.5 | 4.8 | 4.4–5.3 | 4.3 | 3.7–4.8 | 5.0 | 4.4–5.5 | 0.071 |
| Highest quartile | 28.8 | 18.5–39.1 | 30.3 | 19.6–41.1 | 31.6 | 18.1–45.2 | 30.0 | 19.5–40.5 | 0.541 |
Mean values and 95% confidence intervals (95% CI) for (a) serum neurofilament light chain (sNfL) and (b) plasma neurofilament light chain (pNfL).
CI confidence interval, pNfL plasma neurofilament light chain concentration (pg/mL), RT room temperature, sNfL serum neurofilament light chain concentration (pg/mL).
aCalculated by repeated measurement ANOVA.