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. 2021 May 26;12:3167. doi: 10.1038/s41467-021-23227-4

Table 2.

Phytotoxicity tests.

Trophic level: terrestrial
Bioindicators Plant Seeds (ryegrass) Microorganisms (fungi and bacteria)
Samples (at 98 days) Seedling emergence (%) Plant biomass (mg) Net apparent CO2 production rate (g/d)
Blank compost [threshold 90%] 78 ± 15 [70%] 84 ± 28 [76 mg] 0.46 ± 0.02 [0.41 g/d]
Cellulose 89 ± 8 115 ± 30 0.78 ± 0.01
No phytotoxicity No phytotoxicity No ecotoxicity
Sepia Melanin 76 ± 12 77 ± 24 0.61 ± 0.01
No phytotoxicity No phytotoxicity No ecotoxicity
Polyethylene 66 ± 19 48 ± 26 0.49 ± 0.05
No phytotoxicity Potentially phytotoxic No ecotoxicity
Copper (II) phthalocyanine 57 ± 13 50 ± 33 0.40 ± 0.01
No phytotoxicity No phytotoxicity Potential ecotoxicity
Polyphenylene sulfide 42 ± 15 14 ± 6 0.48 ± 0.05
Potentially phytotoxic Potentially phytotoxic No ecotoxicity

Seedling emergence and plant biomass in wet sandy soil (used as a “pristine” or “clean” substratum). After 19 days, the number of plants in this wet sandy soil is 20 ± 0. In addition, a second bioindicator, the compost microbiota (fungi and bacteria), representing a lower trophic level than the plants, was used. Thus, the microbial respiration rate (apparent CO2 production) is also reported in this table, in g/d. As recommended by ASTM, a 90% threshold value is used to validate potential phytotoxicity. Statistical analysis is detailed in SI.