Table 2.
List of samples analyzed in the study and simultaneity of the experimental steps.
| Sample | n | Experiment simultaneity | ||
|---|---|---|---|---|
| DNA extraction | qPCR | 16S rRNA gene profiling | ||
| Blood | 43 | A | A | A |
| Cextr | 4 | B | B | n.p. |
| CdEDTA | 1 | |||
| CvEDTA | 1 | |||
| CqPCR | 4 | B | ||
| Blood | 42 | C | C | C |
| Blood | 1 | D | D | D |
| CdEDTA | 1 | |||
| CvEDTA | 1 | |||
| Cextr | 4 | |||
Samples with the same letter (A, B, C or D) have been concurrently processed. Cextr, ultrapure water; CdEDTA, commercial phosphate buffered saline (PBS) added directly into an EDTA tube; CvEDTA, PBS added into an EDTA tube after passage through a vacutainer blood collection system; n, number of samples; n.p., analysis not performed for the respective samples.