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. 2021 May 26;11:11005. doi: 10.1038/s41598-021-90565-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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FGF2 expression in skeletal muscle of young and old WT muscle. Young (2 months) and old (18 months) WT mouse hindlimb muscles were isolated and qPCR and Western blotting for Fgf2 mRNA and FGF2 protein were performed on whole muscle isolates. (a) Fgf2 mRNA relative to actin. n = 6–10 mice/group. (b) Representative Western blots for FGF2 protein and (c) quantitative analysis of 17.5 kDa FGF2 isoform. (d) Quantitative analysis of 21/22 kDa FGF2 isoforms. Quantifications were summed from 3 independent experiments. *Significantly different from young.