Table 1.
Process assumptions.
| Process assumptions | Annotations & calculations | Value |
|---|---|---|
| Target doses per year | Dose/yr | 5000 |
| Platelets per patient | Dose | 2.4 × 1011 |
| Process efficiency | η | 50% |
| Doses per batch | D/B | 1 |
| Platelets per batch | P/B | 4.8 × 1011 |
| Platelets per Megakaryocyte (MK) | P/MK | 100 |
| MK per batch | MK/D = (P/B)/(P/MK) | 4.8 × 109 |
| IPSC seeding density/cm2 | SDiPSC | 1.2 × 104 |
| Fold expansion (MK per iPSC) | FE | 75 |
| IPSC required per batch for differentiation | iPSCt0 = (MK/D)/FE | 6.4 × 107 |
| Surface area required for differentiation (cm2) | SAdiff = iPSCt0/SDiPSC | 5.33 × 103 |
| Vessel format | 6-well plate | |
| Vessel surface area (cm2) | SAv | 57 |
| Number of vessels start of MK diff | Nvt0 ≈ SAdiff/SAv | 94 |
| Operators at start | Ot0 ≈ Nvt0/36 | 3 |
| Number of isolators at start | ISOt0 ≈ Ot0/2 | 2 |
| Number of vessels at day 12 of MK diff | Nvt12 = 2Nvt0 | 188 |
| Operators at day 12 of MK diff | Ot0 ≈ Nvt12/36 | 6 |
| Number of isolators at day 12 of MK diff | ISOt12 ≈ Ot12/2 | 3 |
| Number of vessels at day 16 of MK diff | Nvt16 = 2Nvt12 | 376 |
| Operators start at day 16 of MK diff | Ot16 ≈ Nvt12/36 | 11 |
| Number of isolators at day 16 of MK diff | ISOt16 ≈ Ot16/2 | 6 |