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. 2021 May 26;11:11023. doi: 10.1038/s41598-021-89389-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptional regulation of PTENP1-AS by C/EBPB. (a) A scheme showing binding of C/EBPB at the PTENP1 locus (data retrieved from the Zenbu FANTOM5 CAGE and the UCSC genome browser) and the predicted DNA binding site for C/EBPB (R is an A/G, Y is C/T). (b) qRTPCR measuring the expression levels of C/EBPB (n > 3). (c) ChIP followed by qRTPCR analysis measuring the levels of C/EBPB at the PTENP1-AS promoter (n = 3). (d) qRTPCR measuring expression levels of C/EBPB and unspliced PTENP1-AS in A375PR1 cells upon siRNA-induced knockdown of C/EBPB (n = 3).