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. 2021 May 26;7:23. doi: 10.1038/s41540-021-00180-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Manual gating of “live” cells using SSC-A and FSC-A. Three gates of different size were included and the percentage of “live” cells for each gate was calculated. b Comparison of precisions and recalls of live cell predictions between the MLP 16-6-based and conventional gating methods. c Classifications of live (green) and apoptotic (yellow) cells using manual gating and MLP 16-6-based gating. The x-axis denotes log₁₀(Alexa Fluor 488), and the y-axis denotes log₁₀(PI).