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. 2021 May 26;11:10997. doi: 10.1038/s41598-021-90517-8

Figure 6.

Figure 6

Analysis of infiltrating cells in spinal cord. (a–f) Cryosections of lumbar spinal cord were stained with Toluidine Blue and analyzed to determine the level of cellular infiltrates. Representative photos of coronal sections of spinal cords obtained from WT and MUT control animals (CFA) showed no cellular infiltrates (a,d). Representative spinal cord images from EAE mice showed typical cellular infiltrates (white arrows) in WT and MUT mice at acute (b,e) and chronic stages (c,f). Scale Bar: 200 μm. (g–j) The level of cellular infiltration in WT and MUT EAE groups was evaluated in meninges and parenchyma, as follows: 0, no infiltrates; 1, one or two cell focus; 2, more than 2 small focuses or one extensive focus; 3, more than two deep or extensive focus of cells. During acute stage, spinal cord from MUT-EAE animals showed higher level of infiltrates in both (g) parenchyma and (h) meninges compared to WT-EAE. In chronic EAE phase, the extents of cellular infiltration in both spinal cord compartments were lower respect to the acute phase; however, no significant differences were observed between genotypes at this stage (i,j). All values are presented as media ± SEM. Results were analyzed using an unpaired t-Student test. *p < 0.05. WT-EAE n = 5; MUT-EAE n = 5.