Table 2.
Outline of the [18F]DPA-714 cassette on the Trasis AllinOne radiosynthesiser, with description of reagent and materials position. Positions outlined in boldface are original parts of the commercially available [18F]FDOPA cassette
| Manifold position | Reagents or materials attached |
| 1 vertical | PE tubing to [18O]H2O recovery vial |
| 1 horizontal | Silicone tubing to pressure inlet |
| 2 | Spike, white Luer Lock ring and barrel for 20 mm vial |
| 3 | 3 mL sterile syringe with Luer Lock |
| 4 | Silicone tubing connected to QMA cartidge in position 5 |
| 5 | Activated QMA cartridge |
| 6 | 3 mL sterile syringe with Luer Lock (activity inlet) |
| 7 | Reactor 6 mL with 2 tubes (male connector to cassette, female to exhaust) |
| 8 | Spike, red Luer Lock ring with barrel for 20 mm vial |
| 9 | 10 mL BD PlastiPak sterile syringe with Luer Lock |
| 10 | Spike, blue Luer Lock ring |
| 11 | PE tubing OD 2 mm length 150 mm, connected to Load port of HPLC unit |
| 12 | Spike, green Luer Lock ring with barrel for 20 mm vial |
| 13 | PE tubing OD 2 mm length 150 mm, connected to 25 mL HPLC product receiving vial via sterile needle |
| 14 | 20 mL HSW Norm-Ject sterile syringe with Luer Lock |
| 15 | PE tubing OD 2 mm length 100 mm, connected to Sep-Pak C18 Plus cartridge in position 17 |
| 16 | 0.9% NaCl for injection bottle, connected to valve using PE tubing OD 2 mm length 150 mm with spike |
| 17 | Activated Sep-Pak C18 Plus cartridge |
| 18 vertical | Tubing to dispensing hot cell |
| 18 horizontal | Silicone tubing to exhaust |
| Other components | Reagents or materials attached |
| Collect port HPLC unit | PE tubing OD 2 mm length 150 mm, connected to 25 mL HPLC product receiving vial via sterile needle |
| HPLC product collection 25 mL vial | Connected to cassette via tubing in Load port and postion 13 |
| QMA reagent for elution (10 mL vial) | Connected to position 2 via spike |
| Precursor solution (10 mL vial) | Connected to position 8 via spike |
| Quench solution (25 mL vial) | Connected to position 10 via spike |
| Ethanol solution (10 mL vial) | Connected to position 12 via spike |
| 0.9% NaCl for injection solution (250 mL bottle) | Connected to position 16 via tubing and spike |