Fig. 5. XAB2 is released from U snRNPs and pre-mRNAs upon DNA damage.
a RNA immunoprecipitation of XAB2 on U4, U5, and U6 snRNAs at 2, 6, and 24 h post-UV irradiation. b RNA immunoprecipitation of XAB2 on pre-mRNAs in untreated and UV-irradiated HEPA cells (as indicated). c bXAB2 pulldowns followed by western blot analysis with FLAG (for bXAB2), PRP8, PRP19, BCAS2, and SNRP40 in UV-irradiated (10 J/m2) and control MEFs. The images shown on Fig. 5c are representative of experiments that were repeated three times. d RNA immunoprecipitation of XAB2 on UsnRNAs in untreated, UV-irradiated cells and UV-irradiated cells pre-treated with inhibitors against ATM (ATMi) and ATR (ATRi; as indicated). e Immunofluorescence detection of UV-induced CPDs in UV-irradiated photolyase transgenic MEFs exposed to 1 h of PR light (CPDs are repaired) or dark (CPDs remain) as indicated. The images shown are representative of experiments that were repeated three times. f RNA immunoprecipitation (RIP) of XAB2 on U4 and U6 snRNAs in UV-irradiated photolyase transgenic MEFs exposed to 1 hour of PR light (CPDs are repaired) or dark (CPDs remain); g RNA immunoprecipitation (RIP) of XAB2 on pre-mRNAs in UV-irradiated photolyase transgenic MEFs exposed to 1 h of PR light (CPDs are repaired) or dark (CPDs remain); h bXAB2 RNA pull downs on U4 and U6 snRNAs in untreated or illudin S-treated BirA and bXAB2 MEFs; i bXAB2 RNA pull downs on U4 and U6 snRNAs in P15 bXAB2 or bXAB2;Csbm/m mouse livers. RIP signals are shown as fold enrichment (F.E) of % input of antibody or bXAB2 over % input of control antibody (IgG) or BirA. All scatter and bar blots in this manuscript are presented as mean ± SEM. P values were calculated by two-tailed Student’s t test, unless otherwise indicated. Error bars indicate SEM among three biological replicates in all cases. Source data provided as a Source Data file.