Figure 2.

(A) Representative TLC of [¹⁴C]-labeled lipids extracted from M. bovis BCG treated with DMSO, positive control BM212 (1 × MIC₉₀, 7.6 μM), negative control ethambutol (4 × MIC₉₀, 12.8 μM), and test compounds 16, 33, and 47 (1 × MIC₉₀, 0.04 μM, 0.08 μM, 3.4 μM) at radiolabeling time points 1 h and 3 h. (B) Fraction of TDM in total mycolates (MA+TMM+TDM) in control and drug-treated samples at time points 1 h and 3 h. For each drug-treated sample, the TDM/total mycolates ratio was normalized against the TDM/total mycolates ratio of DMSO control to give %TDM levels relative to DMSO control. (C) Quantification of TMM levels as described for panel B. Descriptions for panels A, B, and C apply to panels D, E, and F, respectively, except that lipids were extracted from the M. bovis BCG MmpL3 mutant B47-4. Lipids monitored on the chromatograms were TMM, TDM, mycolic acid (MA), phosphatidylethanolamine (PE), cardiolipin (CL), phosphatidylinositol (PI), and phosphatidylinositol mannoside (PIM). Experiments were repeated twice. One representative result is shown.