Table 4. Antimycobacterial Profiles of Indolecarboxamide 16 and Indolylmethylamine 33.

				M. bovis BCG (μM)
	M. tb H37Rv (μM)				MIC50/MIC90 in 7H9 media
no.	MIC50a	MIC90a	MBC99.9b	MBC99.9b	standard media	no glycerol	with 10% FBS	Vero IC50 (μM)c
16	0.02	0.04	0.08	0.08	0.02/0.04	0.01/d	0.02/0.08	>100
33	0.05	0.13	0.63	0.16	0.04/0.08	0.04/d	0.07/0.16	22 ± 2

^aLowest concentration required to reduce bacterial growth by 50% (MIC₅₀) or 90% (MIC₉₀) compared to untreated cultures. MIC₅₀ and MIC₉₀ of isoniazid (control) on M. tb were 1.8 μM and 3.8 μM.

^bMinimum compound concentration required to kill 99.9% of bacteria. Control isoniazid had MBC_99Mtb of 3.2 μM and MBC_99.9BCG of 10 μM.

^cConcentration required to reduce growth of mammalian Vero cells by 50% compared to untreated controls. Selectivity indices (IC_50Vero/MIC₅₀, SIs) for 16 was >5000 (M. tb, M. bovis BCG), and for 33, SI was 366 (M. tb) and 550 (M. bovis BCG).

^dMIC₉₀ could not be accurately determined in 7H9 in the absence of glycerol due to poor bacterial growth.