Table 3. Compositional Information on mRNA-LNPs As Determined by SANSa.
| shell
volume fractions |
core “dry”
volume fractions |
||||||
|---|---|---|---|---|---|---|---|
| sample | DSPCb | Chol | MC3 | DMPE-PEGb | Chol | MC3 | mRNAb |
| MCH | 25.4 | 32.6 ± 0.2 | 39.0 ± 0.8 | 3.0 | 12.4 ± 0.2 | 75.2 ± 0.4 | 12.4 |
| MCHPC | 21.9 | 30.9 ± 0.2 | 44.6 ± 0.8 | 2.5 | 11.1 ± 0.2 | 74.5 ± 0.4 | 14.4 |
| MMCc | 30 | 36 ± 4 | 30.5 ± 1.8 | 3.5 | 12.2 ± 0.5 | 76.8 ± 0.2 | 11.0 |
| average and SD | 26 ± 4 | 33 ± 3 | 38 ± 7 | 3.0 ± 0.5 | 12 ± 1 | 75 ± 1 | 13 ± 2 |
a
Volume fractions estimated from the fitted SLD using the core–shell model. Excellent agreement with the experimental results was obtained, and the initial mixing ratios are within experimental error (see Methods section and the SI, Tables SI4 and SI5 for molar fractions).
b
The distribution of these components was kept fixed: DSPC and DMPE-PEG in the shell and mRNA in the core.
c
The largest error for the composition in MMC relates to higher error in the SLD of the shell (Table SI2).