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. 2021 Apr 19;15(4):7065–7077. doi: 10.1021/acsnano.1c00188

Figure 1.

Figure 1

Scheme and characterization of the DONA structures. (A) Schematic representation of the DNA origami nanofork having a DNA bridge that is 90 nt long. (B) AFM and TEM images of the DNA origami nanofork. The sizes of the close-up AFM and TEM images on the lower left are both approximately 90 nm × 100 nm. (C) Two differently coated nanoparticles can be attached selectively via DNA hybridization to the two different sequences of DNA capture strands on the arms and the bridge of the DNA origami to form DONA structures. A side-view and a top-view scheme of the assembled DONA structures is shown. The position of an analyte molecule attached to the DNA bridge is illustrated as a green glow. The DNA hybridization of nanoparticles and DNA origami in the “zipper” configuration is shown in the insets. (D) Representative TEM image of an assembled smaller gap size Au DONA (more examples shown in Figure S2), along with distributions of gap sizes determined from TEM images of smaller and larger gap size DONAs with an average gap size of 1.17 and 1.40 nm, respectively. The number (N) of the counted DONAs was 109 and 95 for the smaller and larger gap sizes, respectively.