Coarse-grained
oxDNA model of the DNA origami nanofork. (A) Snapshots
of two side views and one top-view of the nanofork with 90 nt DNA
bridge as simulated using the oxDNA model. (B) Plot of the simulated
probability distribution of distance between the tips of the two arms
and comparison with experimental values obtained from AFM images (for
no bridge and 90 nt and 120 nt bridges, the maxima are 41.13, 40.82,
and 41.84 nm, respectively). The number of nanoforks analyzed by AFM
is given in the left legend. (C) Plot of the simulated probability
distribution of the flexibility of the center of a 90 nt and a 120
nt DNA bridge with respect to the straight bridge position, with the
maxima at 1.56 nm and −0.6 nm, respectively.