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. 2021 May 13;12:670054. doi: 10.3389/fphar.2021.670054

FIGURE 6.

FIGURE 6

SGD blunted TLR4/NF-κB signaling pathway in PCOS rats and LPS-stimulated RAW264.7 cells. (A) The mRNA levels of TLR4, PI3K, Akt, and NF-κB p65 in rats ovarian tissues were quantitated by real-time PCR (n = 5). (B) The mRNA levels of TLR4, PI3K, Akt, and NF-κB p65 in RAW264.7 cells were quantitated by real-time PCR (n = 3). (C,D) The protein levels of TLR4, p-PI3K, PI3K, p-Akt, Akt, p-p65, and p65 in rats ovarian tissues were quantitated by Western blotting (n = 3). Data were presented as mean ± SEM. **p < 0.01 vs. normal group, #p < 0.05 and ##p < 0.01 vs. PCOS model group, &&p < 0.01 and &&&p < 0.001 vs. the untreated group, †p < 0.05, ††p < 0.01, and †††p < 0.001 vs. the LPS-stimulated group.