TABLE 1.

List of primers for real-time PCR.

Source	Gene name	Forward primer (5′–3′)	Reverse primer (5′–3′)
	Occludin	GAC​CTT​GTC​CGT​GGA​TGA​CTT​CAG	ATC​AGC​AGC​AGC​CAT​GTA​CTC​TTC
	Claudin-1	ACT​GTG​GAT​GTC​CTG​CGT​TTC​G	GCA​GCA​GCC​CAG​CCA​GTA​AAG
	ZO-1	GAA​GGC​GGA​TGG​TGC​TAC​AAG​TG	AGG​CGA​AAG​GTA​AGG​GAC​TGG​AG
	TLR4	AGA​GAA​TCT​GGT​GGC​TGT​GGA​GAC	AAA​GGC​TTG​GGC​TTG​AAT​GGA​GTC
	PI3K	TCT​GGA​ATG​TGT​GGC​TGG​AGT​TTG	GGA​GGA​GGA​AGC​GGT​GGT​CTA​TC
Rat	Akt	CTC​AAC​AAC​TTC​TCA​GTG​GCA​CAA​TG	GCA​GGC​AGC​GGA​TGA​TGA​AGG
	p65	GAT​GGC​TTC​TAT​GAG​GCT​GAA​CTC​TG	CTT​GCT​CCA​GGT​CTC​GCT​TCT​TC
	TNF-α	AGC​ACG​GAA​AGC​ATG​ATC​CG	ACC​GAT​CAC​CCC​GAA​GTT​CA
	IL-1β	CCC​TTG​TCG​AGA​ATG​GGC​AG	GAC​CAG​AAT​GTG​CCA​CGG​TT
	IL-6	TCT​GCT​CTG​GTC​TTC​TGG​AGT​TCC	GTT​GGA​TGG​TCT​TGG​TCC​TTA​GCC
	IL-18	CAG​CCA​ACG​AAT​CCC​AGA​CC	AGA​TAG​GGT​CAC​AGC​CAG​TCC
	GAPDH	GTC​CAT​GCC​ATC​ACT​GCC​ACT​C	CGC​CTG​CTT​CAC​CAC​CTT​CTT​G
	TLR4	CCG​CTT​TCA​CCT​CTG​CCT​TCA​C	TGC​CGT​TTC​TTG​TTC​TTC​CTC​TGC
	PI3K	AGA​AAG​GTG​TGC​GGC​AGA​AGA​AG TAC​CAC​TAC​GGA​GCA​GGC​ATA​GC	TAC​CAC​TAC​GGA​GCA​GGC​ATA​GC
Mouse	Akt	AGA​GGC​AGG​AAG​AAG​AGA​CGA​TGG	GCA​GGA​CAC​GGT​TCT​CAG​TAA​GC
	p65	ACA​CCT​TCC​CAG​CAT​CCC​TCA​G	CTT​CCG​ACA​GCG​TGC​CTT​CC
	GAPDH	TCA​CCA​TCT​TCC​AGG​AGC​GAG​AC	TGA​GCC​CTT​CCA​CAA​TGC​CAA​AG