FIGURE 1.

Bone marrow non-congenic chimeras and response to chronic P. aeruginosa infection. CF→CF (closed triangles), WT→CF (open triangles; closed bars), CF→WT (closed circles; clear bars) and WT→WT (open circles) mice were inoculated with P. aeruginosa-laden agarose beads on Day 0, three months following bone marrow reconstitution. Mice were monitored daily following infection. Moribund mice were euthanized for humane reasons and included as if spontaneous death had occurred. (A) Significantly different survival rates on Day 10 between the four possible groups (p = 0.03; Fisher’s exact test) where donors and recipients were males. Significantly different survival rates on Day 10 with improvement in survival of CF animals with WT BM and decrease survival of WT mice with CF BM compared to survival of CF animals and WT animals without BM four possible groups (p = 0.0284; Fisher’s exact test). (B) BAL fluid was collected from one subset of WT→CF (N = 11), CF→WT (N = 10) mice sacrificed three days after P. aeruginosa challenge for cytokine and chemokine analysis. *Significantly different from CF → WT mice (p ≤ 0.03; Wilcoxon test). (C) Following BAL fluid collection in mice represented in Figure 1C, the areas of inflammation in the left and right lungs were evaluated using point counting. *Significantly different from CF → WT mice (p ≤ 0.01; Wilcoxon test). (D) The area of the location of the inflammation in the right lung is shown. *Significantly different from CF → WT mice (p = 0.03; Wilcoxon test). (E) The severity of the inflammation within the endobronchial spaces of the right lung is shown. *Significantly different from CF → WT mice (p = 0.04; Wilcoxon test). Data in the bar graphs are shown as the means ± SEM.