. 2021 Apr 5;7(4):613–623. doi: 10.1021/acscentsci.0c01336

Table 1. Representative Proteins Showing Hyperreactivity (R < 2.0) with Scout Fragment Alkyne Probes KB02yne or KB05yne^a.

gene	protein	scout fragment alkyne ratio	IA-alkyne ratio	liganded cysteine	scout
AKR1B10	aldo-keto reductase family 1 member B10	0.6 (KB02yne)	3.2	C299	KB02
COG8	conserved oligomeric Golgi complex subunit 8	1.6 (KB02yne)	3.8	C96	KB02
DUS2L	dihydrouridine synthase 2	1.0 (KB02yne)	3.6	C116	KB02
HELLS	lymphoid-specific helicase	1.5 (KB02yne)	4.9	C836	KB02
WDR45B	WD repeat domain phosphoinositide-interacting protein 3	1.1 (KB02yne)	4.3	C63	KB02
ALG3	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase	1.1 (KB05yne)	3.3	C21	KB05
CDKN2AIP	CDKN2A-interacting protein	1.5 (KB05yne)	6.5	C516	KB05
FAM40A	protein FAM40A	1.4 (KB05yne)	3.4	C769	KB05
SLC4A11	sodium bicarbonate transporter-like protein 11	1.2 (KB05yne)	4.3	C170	KB05
SMARCD1	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1	1.3 (KB05yne)	4.3	C460	KB05

Also shown are cysteine residues in these proteins found in previous chemical proteomic studies^{24,26,27,37,38} to be liganded by the corresponding scout fragments (KB02 or KB05, respectively).

Table 1. Representative Proteins Showing Hyperreactivity (R < 2.0) with Scout Fragment Alkyne Probes KB02yne or KB05ynea.

Table 1. Representative Proteins Showing Hyperreactivity (R < 2.0) with Scout Fragment Alkyne Probes KB02yne or KB05yne^a.