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. 2021 Mar 23;7(4):688–696. doi: 10.1021/acscentsci.1c00200

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© 2021 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Gene expression analysis of cluster 1 genes and cytokine analysis confirm GMTP’s reactivity. (A) Gene expression and statistical analysis of qRT-PCR data for Il6, Acod1, Cxcl9, Lcn2, and Isg15 in BMDMs treated with 0, 20, or 100 μM 3, 4, or 5 or control (water) for 18 h. Total RNA was harvested, and the expression levels of selected genes were analyzed by qRT-PCR. Data were analyzed using one-way ANOVA and the ordinary ANOVA test. Error bars represent mean ± SD. (B) IL6 expression in the supernatant of BMDMs from (A) after 18 h of stimulation with the indicated peptidoglycan fragments and concentrations. Data were analyzed using one-way ANOVA and the ordinary ANOVA test. Error bars represent mean ± SD.