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. 2021 May 13;9:673231. doi: 10.3389/fcell.2021.673231

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Copyright © 2021 Bai, Ren, Liu, Li, Guan and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EV-carried microRNAs promote the differentiation of i-Beta cells from iPSCs. (A) Gene expression of iPSCs at various stages of differentiation compared with control iPSCs. Data are expressed as the mean ± SD of three independent experiments (n = 3). ^∗p < 0.05, ^∗∗p < 0.001 relative to control iPSCs. (B) The abundances of the top 10 miRNAs in EVs were verified by qPCR and expressed as the fold change versus the external control (n = 3). (C) Flow cytometric analysis of insulin expression during each stage of differentiation. Five miRNAs were selected from the top 10 miRNAs to analyze their roles in the formation of i-Beta cells from iPSCs. Insulin-positive cells were significantly decreased after addition of miR-212-3p and miR-132-3p inhibitors. in: miRNA inhibitor.