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. 2021 May 13;9:671841. doi: 10.3389/fcell.2021.671841

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Copyright © 2021 Chu, Taghizadeh, Vazquez-Armendariz, Herold, Chong, Chen, Zhang, El Agha and Bellusci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the labeling of FGF10^Pos cells at E15.5. (A) Fgf10^Ki-v2/+ were crossed with Fgf10^+/+; tdTom^flox/flox mice. Pregnant females received a single IP injection of tamoxifen when the embryos were at E15.5 and sacrificed at E18.5. (B) Head, limbs, lung and cecum of Fgf10^+/+; tdTom^flox/+ and Fgf10^Ki-v2/+; tdTom^flox/+ embryos are shown. Note the expression in the external ear (arrow). (C) Higher magnification of lung and trachea showing enriched tdTomato expression in the interlobular septa and the presence of tdTomato expression between the cartilage rings, respectively. Scale bar in (B): head: 1.5 mm, Limb: 0.75 mm, Lung: 0.5 mm, Cecum: 0.1 mm. Scale bar in (C): Lung 25 μm, Trachea 125 μm.