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. 2021 May 13;12:670122. doi: 10.3389/fimmu.2021.670122

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Copyright © 2021 Weng, Li, Li, Liu, Chen and Shiao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptional Analysis of Immune-Responsive Genes in the Mosquito Midgut. The relative mRNA levels of the immune-responsive genes from three and seven days after normal (BF) and infectious DENV2 blood-fed (DENV2) mosquito midguts were examined with qRT-PCR analysis. Midguts of mosquitoes fed with non-infectious blood were used as controls. An equal amount of total RNA from each group was used for cDNA synthesis. Ribosomal protein S7 was used for normalization of relative target gene mRNA expression. Values are mean ± S.E. (error bars) of the copy number of each gene. At least three biological cohorts from each time point were used for analysis.