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. 2021 May 13;12:670122. doi: 10.3389/fimmu.2021.670122

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Copyright © 2021 Weng, Li, Li, Liu, Chen and Shiao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of Defensins and Cecropins in Thioester-Containing Protein 1 (TEP1) Transgenic Mosquitoes after Blood Meals. (A–D) The whole bodies of female wild type or transgenic mosquitoes (AaCPA>2miR-TEP1) were collected 6, 12, 24, 48, and 72 hours after a normal blood meal. Total RNA was extracted and quantified, followed by cDNA synthesis and subjected to quantitative real-time PCR analysis with a specific primer for Rel1 (A), Rel2 (B), defensins (C), and cecropins (D). At least three biological cohorts from each time point were used for analysis. Ribosomal protein S7 was used for normalization of relative target gene mRNA expression. PE means post-eclosion.