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. 2021 May 13;9:658966. doi: 10.3389/fcell.2021.658966

FIGURE 4.

FIGURE 4

TRIM71 controls proliferation in the in vitro PGC-like model cell line TCam-2. (A) RT-qPCR measurement of TRIM71 in wild type (siCtrl) and TRIM71 KD (siTRIM71 #1 and siTRIM71 #2) TCam-2 cells 72 h post-transfection (n = 3). (B) Proliferation of wild type (siCtrl) and TRIM71 KD (siTRIM71 #1 and siTRIM71 #2) TCam-2 cells depicting the increase in cell numbers over time after transfecting them with siRNAs and seeding them in equal numbers (d0) (n = 5). (C) Area under curve (AUC) measurements for the proliferation curves depicted in B (n = 5). (D–G) RT-qPCR measurements for the indicated PGC markers in wild type (siCtrl) and TRIM71 knockdown (siTRIM71 #1 and siTRIM71 #2) TCam-2 cells 72 h post-transfection (n = 3–6). For all RT-qPCRs, the housekeeping gene 18S rRNA was used for normalization. For all graphs, error bars represent the SEM. ***P-value < 0.005, **P-value < 0.01 (unpaired Student’s t-test). See also Supplementary Figure 8.