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. 2021 May 13;9:658966. doi: 10.3389/fcell.2021.658966

FIGURE 5.

FIGURE 5

NCCIT cells with TRIM71 mutations show population maintenance defects in growth competition assays. (A) Pie charts showing allele frequencies for wild type (EV) and TRIM71 RING mutant (ΔRING) or (B) TRIM71 NHL mutant (ΔNHL6) NCCIT cells obtained at different time points during growth competition assays. For the assay, NCCIT wild type cells (EV) were mixed 1:1 with either TRIM71 RING mutant (ΔRING) or TRIM71 NHL mutant (ΔNHL6) NCCIT cells and directly analyzed (d0) before their culturing, following by subsequent analysis at several time points (d3, d7, d14, and d21). Allele frequency was analyzed via NGS using the Illumina MiSeq platform. For TRIM71 mutant alleles, reads with in-frame mutations and frameshift mutations (loss-of-function) in each respective domain (RING or NHL) are depicted. The total number of sequencing reads for each time point is indicated under each respective pie chart. See also Supplementary Figures 8–10.