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. 2021 May 13;9:646646. doi: 10.3389/fcell.2021.646646

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Copyright © 2021 Myneni, Szalayova and Mezey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Femur epiphysis and metaphysis have lower erythroid precursors compared to the diaphysis. (A) Percentage of Ter119⁺ cells in epi and metaphysis, and diaphysis of the femur. (B) Ratio of Ter119⁺ cells to BM cells. (C) Representative FACS plots showing expression of BM Ter119⁺ erythroid precursors from epi and metaphysis and diaphysis based on CD44 expression and size (FSC-forward scatter). The gated region shows percentage of erythroid precursor cells. (D) Erythroid precursors within Ter119⁺ cells from epi and metaphysis and diaphysis. (E) Ratio of erythroid precursors to BM cells. n = 4 mice in two independent experiments. Data represented as mean ± SD; Statistical significance was assessed using an unpaired t test. *p < 0.05.