Table 1.
The diagnostic information of high-throughput 16S rDNA sequencing and bacterial culture.
| No. | Gender | Age | Conjunctival swabs | Corneal scrapings | Whether two sequencing results is same | Whether sequencing results same to culture | ||
|---|---|---|---|---|---|---|---|---|
| 16S rDNA sequencing | Culture | 16S rDNA sequencing | Culture | |||||
| 1 | Female | 61 | f_Enterobacteriaceae g_Escherichia-Shigella (2.5%) |
– | f_Moraxellaceae (2.3%) |
– | No | No |
| 2 | Female | 69 | g_Sphingomonas (1.5%) |
– | g_Sphingomonas (1.6%) |
g_Micrococcus | Yes | No |
| 3 | Male | 80 | g_Solanum torvum s_Solanum torvum (3.3%) |
– | g_Bacillus (6.7%) |
– | No | No |
| 4 | Male | 52 | f_Moraxellaceae (2.7%) |
f_Enterobacteriaceae g_Proteus s_Proteus mirabilis |
f_Bacteroidaceae g_Bacteroides s_Bacteroides vulgatus (5.2%) |
f_Enterobacteriaceae g_Proteus s_Proteus mirabilis |
No | No |
| 5 | Female | 65 | g_Alloprevotella (1.2%) |
– | g_Pseudoxanthomonas (5.9%) |
– | No | No |
| 6 | Female | 38 | g_Bacillus (7.2%) |
– | g_Escherichia-Shigella (10.7%) |
– | No | No |
| 7 | Male | 35 | g_Enterococcus (8.1%) |
– | g_Prevotella (11.0%) |
– | No | No |
| 8 | Male | 66 | g_Escherichia-Shigella (2.2%) |
– | g_Bacteroides (3.5%) |
g_Streptococcus s_Streptococcus pneumoniae |
No | No |
| 9 | Male | 67 | g_Escherichia-Shigella (7.3%) |
– | g_Faecalibaculum (11.4%) |
– | No | No |
| 10 | Male | 67 | g_Prevotella (13.2%) |
– | g_Escherichia-Shigella (4.1%) |
– | No | No |
| 11 | Female | 67 | g_Escherichia-Shigella (3.2%) |
– | g_Bacillus (8.2%) |
– | No | No |
| 12 | Female | 51 | g_Staphylococcus s_Staphylococcus warneri (97.9%) |
– | g_Staphylococcus s_Staphylococcus warneri (96.3%) |
– | Yes | No |
| 13 | Female | 73 | g_Moraxella (98.5%) |
g_Staphylococcus s_Staphylococcus epidermidis |
g_Moraxella (99.6%) |
g_Staphylococcus s_Staphylococcus epidermidis |
Yes | No |
| 14 | Male | 57 | g_Pseudomonas (86.8%) |
– | g_Pseudomonas (88.3%) |
– | Yes | No |
| 15 | Male | 24 | g_Pseudomonas (64.4%) |
– | g_Pseudomonas (92.2%) |
g_Staphylococcus s_Staphylococcus epidermidis |
Yes | No |
| 16 | Male | 43 | g_Pseudomonas (65.5%) |
– | g_Pseudomonas (87.6%) |
– | Yes | No |
| 17 | Female | 67 | g_Pseudomonas (67.5%) |
– | – | – | No | No |
| 18 | Female | 80 | g_Pseudomonas (69.4%) |
– | g_Pseudomonas (82.1%) |
– | Yes | No |
| 19 | Male | 63 | – | – | f_Neisseriaceae (42.3%) |
– | No | No |
| 20 | Female | 45 | g_Pseudomonas (75.1%) |
– | g_Pseudomonas (82.0%) |
– | Yes | No |
| Positive rate (coincidence rate) |
95% | 10% | 95% | 25% | 40% | 0% | ||
f, at the family level; g, at the genus level; s, at the species level; the percentage in brackets represents the relative content of the dominant species.