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. 2021 May 13;12:681424. doi: 10.3389/fphar.2021.681424

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Copyright © 2021 Zhang, Zheng, Wang, Lin, Wang, Liu, Yan, Chen, Yang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VHH-0031 prevents proliferation of mesangial cells under high glucose condition via JAK2/STAT3 pathway (A) MTT method was performed to measure the cell viability after incubation of different doses of VHH-0031 (0∼18pM) in condition of normal glucose (NG, 5.5 mM D-glucose) (B,C) Detection of cell proliferation by MTT. Cells cycle were measured by flow cytometry. Cells were treated with (D) normal glucose (NG, 5.5 mM D-glucose) (E) high glucose (HG, 25 mM D-glucose), or (F–H) high glucose (HG, 25 mM D-glucose) in the presence of VHH-0031 (1.7, 5, and 15 pM) for 48 h (I)Western blot were employed to detected the protein of Cyclin D1, Cyclin E1, IL-6, JAK2 and STAT3. Quantitative analysis of Cyclin D1 (J), Cyclin E1 (K), IL-6 (L), JAK2 (M), and STAT3 (N) in HBZY-1. ***p < 0.001 vs NG group. ^### p < 0.001 vs HG group.