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. 2021 May 13;12:652782. doi: 10.3389/fimmu.2021.652782

Figure 5.

Figure 5

PRX3 regulates the NLRP3 inflammasome through mitochondrial ROS after APAP overdose. Primary KCs and hepatocytes were cotransfected with PRX3- and NLRP3-specific siRNAs following APAP challenge. (A) Kupffer cellular supernatant levels of LDH were assessed, n=6. (B) NLRP3, GSDMD-N, caspase-1 p20, IL-1β and IL-18 protein levels in primary KCs n=3. **P<0.01. (C) Hepatocellular supernatant levels of LDH were assessed, n=6. (D) NLRP3, GSDMD-N, caspase-1 p20, IL-1β and IL-18 protein levels in primary hepatocytes, n=3. **P<0.01. PRX3 suppression was applied in primary hepatocytes via PRX3-specific siRNA transfection and subsequent Mito-TEMPO (0.2 mM) treatment before APAP intervention. (E) Representative fluorescence images of MitoSOX, 50 μm. (F) hepatocellular supernatant levels of LDH were assessed, n=6. (G) NLRP3, GSDMD-N, caspase-1 p20, IL-1β, and IL-18 protein levels in primary hepatocytes, n=3. **P<0.01.