FIGURE 1.

Identification of EPCs from human peripheral blood and exosomes from EPCs. The isolated cells were cultured and then collected for detection of CD34, CD133, and VEGFR2 expressions by immunofluorescence staining (A) and flow cytometry (B). Additionally, the phagocytic ability of the cells was examined by dual immuno-staining of Dil-Ac-LDL and FITC-UEA-1 (C). The morphology, size, and concentration of the isolated exosomes were identified by transmission electron microscope and Nanosight tracking analysis, scale bar = 100 nm (D). The expressions of exosome-associated proteins, including Alix, CD63, and TSG101 were detected by western blot (E). EPCs, endothelial progenitor cells. N = 3.