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. 2021 May 13;9:647763. doi: 10.3389/fcell.2021.647763

FIGURE 2.

FIGURE 2

Exosomes from EPCS enhanced angiogenesis and proliferation of HCFs. In the HCFs treated with control (PBS), cell supernatant (CS), or exosomes from EPCs (Exo), the transcriptional expressions of ACTA2, CD31, VE-Cadherin (VE-Cad), and VEGFR2 were determined by qRT-PCR (A), and the expressions of α-SMA, CD31, VE-Cad, and VEGFR2 were determined by western blot (B) and immunofluorescence staining (C). Additionally, the angiogenesis was examined by tube formation assay (D), and the cell proliferation was determined by a fluorescent probe, PKH67 (E). The cells were treated with exosomes (4 μg/ml) of EPCs for 24 h. N = 3. *P < 0.05 and **P < 0.01 vs. control group. HCFs, human cardiac fibroblasts.