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. 2021 May 13;12:666356. doi: 10.3389/fimmu.2021.666356

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Copyright © 2021 Morales-Lange, Ramírez-Cepeda, Schmitt, Guzmán, Lagos, Øverland, Wong-Benito, Imarai, Fuentes, Boltaña, Alcaíno, Soto and Mercado

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Splenocytes of Atlantic salmon. (A) Mononuclear fraction. Black arrows showed cells with morphology associated with monocytes, while red arrows showed examples of cells with lymphocyte-associated morphology. The image was taken with magnification 400×. (B) Flow cytometry (FS-A/SSC-A and histogram/FS-A) using splenocytes at 1 day of culture. (C) Flow cytometry (FS-A/SSC-A and histogram/FS-A) using splenocytes at 15 day of culture.