Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 13;12:666356. doi: 10.3389/fimmu.2021.666356

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Morales-Lange, Ramírez-Cepeda, Schmitt, Guzmán, Lagos, Øverland, Wong-Benito, Imarai, Fuentes, Boltaña, Alcaíno, Soto and Mercado

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Detection of cell-surface markers at the protein level. (A) Confocal microscopy of cell-surface markers in splenocytes of Atlantic salmon cultured for 15 days. Top panels (left and right), cells without IFNγ (control). Lower panels (left and right), cells with IFNγ as inducer. Left panels: in red, CD80/86. Right panels: in red, MHCII. CD83 shown in blue, in all panels. In green (in all panels) Syto9 as a nuclear contrast. Images were taken using 800x amplification. (B) Flow cytometry in splenocytes cultured for 15 days (Side Scatter-Area versus fluorescence). Top panels: Control group without inducer. Lower panels: IFNg group. +Cells: Percentage of positive cells for the corresponding marker.