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. 2021 May 13;12:666356. doi: 10.3389/fimmu.2021.666356

Table 2.

List of primers used for gene amplification by quantitative PCR.

Gene Primer Sequence Tm Reference
cd80/86 Forward GATGTTGAGTGGAGCCTGAA 55°C GenBank: CAQ51440.1
NCBI Gene ID: 106613207
Reverse GACGACAGAGAACAGCATAGAG 55°C
mhc ii Forward AATCAGAGTGACCTGGTTGAG 55°C GenBank: CAD27720.1
NCBI Gene ID: 106565699
Reverse GTGGGAGAGGATCTGGTAGTA 55°C
cd83 Forward AGGATTCGGTTCTGAGATGTAAAG 55°C GenBank: ABC68619.1
NCBI Gene ID: 100136479
Reverse GTGACAGCCTCTTCATCAGTAG 55°C
zbtb46 Forward CCTCTACTGCCAGGTTAAGAAAG 55°C NCBI Reference Sequence: XM_014134725.1
Reverse CAGAGTACATGAAGTCCAGGATG 55°C
ifnγ Forward ATGCTGCTCAGTTCACATCA 61°C NCBI Reference Sequence: NM_001171804.1
Reverse ACGTCCAGAACCACACTCAT 61°C