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. 2021 May 13;9:601600. doi: 10.3389/fcell.2021.601600

FIGURE 4.

FIGURE 4

miR-9 loss-of-function inhibits NSC differentiation. (A) NSCs transfected with either antogomiR-C or antogomiR-9 were cultured for 6 days in differentiation conditions. The transfection efficiency was determined by quantifying the intracellular miR-9 expression levels via RT-qPCR analysis. (B) Representative images of pre-mature cell markers (Tuj1 and GFAP) staining were shown. Proportions of cells exhibiting immunoreactivities of pre-mature cell markers (Tuj1+ and GFAP+) were determined (in the right panel). (C) Representative images of matured cell markers (Map2 and Glast) staining were shown. Proportions of cells exhibiting immunoreactivities of pre-mature cell markers (Map2+ and Glast+) were determined (in the right panel). (D) The expression levels of transcripts corresponding to pre-mature cell markers (βIII-tubulin and GFAP) and matured cell markers (Map2 and GS) was determined by RT-qPCR analysis. Data were represented as mean ± SE from three independent experiments. * and ** denote p < 0.05 and p < 0.01, respectively. Scale bar 100 μm in panel (B,C).