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. 2021 May 13;12:655726. doi: 10.3389/fphar.2021.655726

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Copyright © 2021 Li, Lei, Zhao, Hou, Wang, Xu, Lin, Li, Tang, Yu and Meng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of propofol on PKC knockdown and overexpression in H9C2 cells. Expression of PKC mRNA (A) and protease (B,C) in H9C2 cells transfected with PKC and scramble siRNA. Levels of NRF2 (D) and HO-1 (E) mRNAs in H9C2 cells transfected with a scramble and PKC siRNA. (F). Influence of propofol on H9C2 cell viability of PKC knockdown under oxidative stress condition. (G). Viability of H9C2 cells treated with phorbol 12-myristate 13-acetate (PMA, 0∼4 µM). (H,I). Expression of PKC in H9C2 cells treated with 0.5 µM PMA based on western blot assay. (J–N). Influence of propofol on H9C2 cell PKC, NRF2 and HO-1 expressions of PKC overexpression under oxidative stress condition. Data are expressed as the mean ± SD. Significance was calculated using the ANOVA or Student’s t-test. p < 0.05 was considered statistically significant. *p < 0.05, **p < 0.01, ***p < 0.001.