TABLE 1.

Summary of literature to date presenting microbial production of (R)-1,3-BDO from different substrates.

Highest titre of (R)-1,3-BDO [g/l]	Organism	Substrate	Notes	References
49.5	E. coli JM109	4-Hydroxy-2-butanone with 2-propanol as co-substrate	Plasmid borne overexpression of Leifsonia alcohol dehydrogenase	Itoh et al., 2007
38.7	Candida krusei ZJB-09162	4-Hydroxy-2-butanone with glucose co-substrate	Optimised preparative scale bioconversion process	Zheng et al., 2012
38.3	Pichia jadinii HBY61	4-Hydroxy-2-butanone with glucose co-substrate	Optimised preparative scale bioconversion process	Yang et al., 2014
15.6	E. coli MG1655 lacIq	Glucose	Strain from Kataoka et al. (2013)_ in optimised fed batch fermentation	Kataoka et al., 2014
9.05	E. coli MG1655 lacIq	Glucose	Plasmid borne overexpression of phaA and phaB from Ralstonia eutropha NBRC 102504 and bldfrom C. saccharoperbutylacetonicum ATCC 27012 in optimised batch fermentation	Kataoka et al., 2013
2.4	E. coli BL21	Glucose	Plasmid borne expression of pyruvate decarboxylase (Zymomonas mobilis) and the deoxyribose-5-phosphate aldolase (Bacillus halodurans) in two stage pH controlled bioreactor fermentation	Nemr et al., 2018
1.1	E. coli LMSE51C	Glucose	Aerobic pH controlled fed-batch fermentation of strain from Nemr et al. (2018) with BH1352 harbouring mutations F160Y/M173I	Kim et al., 2020