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. 2021 May 13;11:656564. doi: 10.3389/fonc.2021.656564

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Copyright © 2021 Pan, Diao, Zhong, Wang, Wen and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of cancer cell subclusters PDAC cancer cells. (A) Cell clusters of primary and metastatic PDAC cancer cells were visualized by spilt UMAPs. (B) Percentage of the primary and metastatic cancer cells in each cluster. (C) Heatmap of top 50 genes of each cluster. (D) Presenting of relative expression of representative top genes of each cancer cell cluster. Notably, lincRNAs, LINC01133, MEG3 and NEAT1, were the top genes of Clusters 0, 1 and 2, respectively. Cluster 3 exhibited significantly increased expression of proliferating marker genes.