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. 2021 May 13;11:656564. doi: 10.3389/fonc.2021.656564

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Copyright © 2021 Pan, Diao, Zhong, Wang, Wen and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pseudotime analysis of cancer cell clusters with Slingshot. (A) Cancer cells were clustered under Slingshot packages and similar clusters were identified. (B, C) Inferred trajectory (B) and principal curves of each lineage (C) were visualized by UMAP. (D) Cluster information from Seurat were mapped to Slingshot and both analysis assays were matched. (E) Pseudotime values for each cancer cell cluster were visualized by UMAP. Color depth of each cell/cluster were correlated with pseudotime values.