. 2021 May 13;12:587431. doi: 10.3389/fmicb.2021.587431

TABLE 4.

Induction of defense related enzymes in chili seedlings inoculated with antagonistic rhizobacterial isolates.

Trt.	PO activity (Katal/mg of total proteins)			PPO activity (Katal/mg of total proteins)			PAL activity (Katal/mg of total proteins)
	1 DAI	3 DAI	5 DAI	1 DAI	3 DAI	5 DAI	1 DAI	3 DAI	5 DAI
NC	0.23 ± 0.03^c	0.37 ± 0.03^c	0.33 ± 0.03^c	0.13 ± 0.03^f	0.30 ± 0.06^d	0.40 ± 0.06^e	0.23 ± 0.03^a	0.37 ± 0.07^d	0.40 ± 0.06^e
Flavobacterium spp.	0.37 ± 0.03^ab	1.06 ± 0.05^a	1.19 ± 0.05^a	0.43 ± 0.03^bc	1.50 ± 0.06^b	1.77 ± 0.19^b	0.36 ± 0.02^ab	1.10 ± 0.21^bc	1.38 ± 0.10^cd
B. megaterium	0.33 ± 0.03^bc	0.93 ± 0.03^a	1.17 ± 0.03^a	0.45 ± 0.08^ab	1.33 ± 0.09^b	1.30 ± 0.06^c	0.44 ± 0.07^a	1.33 ± 0.30^abc	1.67 ± 0.14^abc
P. putida	0.33 ± 0.03^bc	0.70 ± 0.06^b	0.87 ± 0.03^b	0.23 ± 0.03^def	1.53 ± 0.12^b	1.57 ± 0.15^bc	0.43 ± 0.15^a	1.42 ± 0.10^ab	1.46 ± 0.10^bc
B. cereus	0.30 ± 0.06^bc	0.67 ± 0.03^b	0.86 ± 0.02^b	0.20 ± 0.06^ef	1.57 ± 0.22^b	1.61 ± 0.13^bc	0.45 ± 0.01^a	1.53 ± 0.23^ab	1.75 ± 0.14^ab
B. subtilis	0.47 ± 0.03^a	1.03 ± 0.03^a	1.17 ± 0.03^a	0.60 ± 0.06^a	2.03 ± 0.15^a	2.43 ± 0.24^a	0.53 ± 0.09^a	1.70 ± 0.21^a	1.90 ± 0.21^a
B. cereus	0.27 ± 0.03^bc	0.63 ± 0.07^b	0.78 ± 0.04^b	0.33 ± 0.09^bcde	1.47 ± 0.09^b	1.52 ± 0.07^bc	0.40 ± 0.04^ab	1.53 ± 0.07^ab	1.58 ± 0.02^abc
P. putida	0.27 ± 0.03^bc	0.67 ± 0.07^b	0.81 ± 0.01^b	0.37 ± 0.03^bcd	1.67 ± 0.15^ab	1.77 ± 0.05^b	0.46 ± 0.03^a	1.23 ± 0.07^abc	1.40 ± 0.03^bcd
P. libanensis	0.27 ± 0.07^bc	0.77 ± 0,03^b	0.88 ± 0.04^b	0.22 ± 0.02^def	1.40 ± 0.17^b	1.33 ± 0.09^c	0.38 ± 0.04^ab	1.26 ± 0.25^abc	1.56 ± 0.19^abc
UC	0.29 ± 0.07^bc	0.41 ± 0.06^c	0.42 ± 0.04^c	0.29 ± 0.05^cde	0.69 ± 0.08^c	0.79 ± 0.05^d	0.43 ± 0.03^a	0.87 ± 0.06^cd	1.06 ± 0.12^d
LSD	0.132	0.145	0.105	0.155	0.376	0.368	0.189	0.524	0.367

Defense related enzymes viz., peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) were studied. Sterilized soil was flooded with sporangial suspension of P. myriotylum before transplanting the chili seedlings. Fifteen days old chili seedlings were inoculated with rhizobacterial suspension (10⁸ cfu/mL) for 2 h before transplanting in sick soil containing pots. Root samples were taken 1, 3, and 5 days after transplant. Only P. myriotylum treated pots were considered as negative control (NC) while non-inoculated plants with pathogen were kept us untreated control (UC). Means are an average of three repeats and (±) indicate the standard error (SE). DAI: days after inoculation.