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. 2021 May 25;20:15330338211019376. doi: 10.1177/15330338211019376

Figure 2.

Figure 2.

Effect of SNHG3 in the regulation of proliferation and metastasis of lung cancer cells. A, Quantitative PCR detection of the silencing effect of siRNA on SNHG3 in A549 and HCC827 cells. B and C, Detection of the proliferation of tumor cells transfected with siRNA using CCK-8. D and E, Detection of the migration and invasion ability of tumor cells transfected with siRNA using Transwell cell migration assay. The cells were stained with DAPI and observed under fluorescence microscope (Magnification, 200×; Scale bar, 50 µm). F and G, Quantification of the relative migration and invasion ability of cells in each group by histogram through counting under different visual fields. H, Analysis of the relative expression of EMT related proteins after siRNA transfection using western blot; GAPDH was used as the internal reference. si, siRNA; si#1 and si#2, two different siRNA duplexes against SNHG3. One-way ANOVA with Tukey post hoc analysis. *P < 0.05 and **P < 0.01.