Table 1.
A summary of monoclonal antibodies targeting the SARS-CoV-2 or SARS-CoV S protein with the 3D experimental structures of their complexes available in PDB
| Protein or antibody | Target | Kd (nM)/Method | PDB ID | Resolution (A)˚ |
|---|---|---|---|---|
| ACE2 | SARS-CoV-2 RBD | 1.2 / BLI (70) 34.6 / BLI (82) 15.2 / BLI (65) |
6M0J (39) | 2.45 |
| S309 | SARS-CoV-2 RBD | IgG = 0.104 Fab = 1.98 / BLI (49) | 6WPS, 6WPT (49) | 3.10, 3.70 |
| CR3022 | SARS-CoV-2 RBD | IgG = 6.28 / BLI (65) IgG < 0.1 Fab = 115 / BLI (88) |
6W41 (88) | 3.08 |
| CB6 | SARS-CoV-2 RBD | IgG = 2.49 / SPR (60) | 7C01 | 2.85 |
| P2B-2F6 | SARS-CoV-2 RBD | IgG = 5.14 /SPR (36) | 7BWJ (36) | 2.85 |
| B38 | SARS-CoV-2 RBD | IgG = 70.1 /SPR(85) | 7BZ5 (85) | 1.84 |
| H11-D4 | SARS-CoV-2 RBD | NA | 6Z43 | 3.30 |
| BD23 | SARS-CoV-2 RBD | NA | 7BYR (8) | 3.84 |
| ACE2 | SARS-CoV RBD | 5.0 / BLI (70) 325.8 / BLI (82) 1.70 / BLI (61) |
3D0G | 2.80 |
| CR3022 | SARS-CoV RBD | IgG < 0.1 Fab = 1 / BLI (88) | NA | NA |
| S309 | SARS-CoV RBD | IgG = 0.12 Fab = 1.81 / BLI (49) | NA | NA |
| m396 | SARS-CoV RBD | IgG = 0.005 Fab = 20 / BLI (50) | 2DD8 (50) | 2.30 |
| S230 | SARS-CoV RBD | IgG = 0.06 / BLI (73) | 6NB6 (73) | 4.30 |
| VHH-72a | SARS-CoV RBD | IgG = 1.15 / SPR (81) | 6WAQ (81) | 2.20 |
| 80R | SARS-CoV RBD | IgG = 1.59 / BLI (61) | 2GHW (34) | 2.30 |
| F26G19 | SARS-CoV RBD | Fab = 26 / SPR (47) | 3BGF (47) | 3.00 |
a
The binding affinity of VHH-72 with the SARS-CoV-2 RBD is Fab = 54 nM.
Abbreviations: ACE2, angiotensin-converting enzyme 2; BLI, biolayer interferometry; Fab, fragment, antigen-binding; NA, not applicable; PDB, Protein Data Bank; RBD, receptor-binding domain; S, spike; SARS-CoV, severe acute respiratory syndrome coronavirus; SPR, surface plasmon resonance.