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. 2021 May 25;218(8):e20201708. doi: 10.1084/jem.20201708

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© 2021 Chen et al.

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Figure 3. — Loss of SERCA2 and SERCA3 disrupts Ca²⁺ homeostasis. (A–D) Flow-cytometric analysis of WT abl preB cells expressing ER-GCaMP6-150 (A) or incubated with Fluo-3 AM Ca²⁺ indicator (C) without further treatment (black histogram), treated with thapsigargin (TG; red histogram), or after bulk inactivation of Atp2a2 and Atp2a3 genes (green histogram). Median fluorescence intensity was calculated and normalized to that of the black histogram in each experiment to derive the relative intensity of ER-GCaMP6-150 (B; mean ± SD; n = 4) and Fluo-3 dye (D; mean ± SD; n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with the untreated sample. (E) Western blot analysis of XBP1s in imatinib-treated WT abl preB cells bulk Hspa5 (gHspa5) inactivated or Atp2a2^−/− cells bulk Atp2a3 (gAtp2a3) inactivated (n = 3). (F) Flow-cytometric analysis of GFP expression from pMGINV rearrangement in cells from E treated with imatinib for 0, 2, or 4 d. (G) Mean ± SD of three pMGINV experiments performed as in F. **, P < 0.01; ***, P < 0.001. n.s., not significant.